Chemical modification strategies for viscosity-dependent processing of gellan gum
Gering, Christine; Rasheed, Anum; Koivisto, Janne; Parraga Meneses, Jenny; Tuukkanen, Sampo; Kellomäki, Minna (2021-10)
Gering, Christine
Rasheed, Anum
Koivisto, Janne
Parraga Meneses, Jenny
Tuukkanen, Sampo
Kellomäki, Minna
10 / 2021
118335
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202107096274
https://urn.fi/URN:NBN:fi:tuni-202107096274
Kuvaus
Peer reviewed
Tiivistelmä
Recently, the hydrogel-forming polysaccharide gellan gum (GG) has gained popularity as a versatile biomaterial for tissue engineering purposes. Here, we examine the modification strategies suitable for GG to overcome processing-related limitations. We emphasize the thorough assessment of the viscoelastic and mechanical properties of both precursor solutions and final hydrogels. The investigated modification strategies include purification, oxidation, reductive chain scission, and blending. We correlate polymer flow and hydrogel forming capabilities to viscosity-dependent methods including casting, injection and printing. Native GG and purified NaGG are shear thinning and feasible for printing, being similar in gelation and compression behavior. Oxidized GGox possesses reduced viscosity, higher toughness, and aldehydes as functional groups, while scissored GGsciss has markedly lower molecular weight. To exemplify extrudability, select modification products are printed using an extrusion-based bioprinter utilizing a crosslinker bath. Our robust modification strategies have widened the processing capabilities of GG without affecting its ability to form hydrogels.
Kokoelmat
- TUNICRIS-julkaisut [17098]