Production of Storage Lipids from Nitrogen-rich Substrate
Kalinainen, Niko Aleksi (2018)
2018
Ympäristö- ja energiatekniikka
Teknis-luonnontieteellinen tiedekunta - Faculty of Natural Sciences
This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.
Hyväksymispäivämäärä
2018-06-06
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tty-201805221780
https://urn.fi/URN:NBN:fi:tty-201805221780
Tiivistelmä
Acinetobacter baylyi ADP1 is a bacterium capable of producing wax esters (WE) in nitrogen limited conditions. WEs are esters of fatty acids and fatty alcohols and they are used in e.g. cosmetics. Many fields of industry produce nitrogen-rich wastes. The utilization of these wastes would be a good objective.
The purpose of the thesis was to study whether ADP1 can genetically modified to produce WEs more efficiently in nitrogen-rich conditions. In order to achieve more efficient production, a plasmid with a gene for a cytoskeleton-binding toxin (cbtA) from Escherchia coli and with an arabinose inducible promoter was constructed and then transformed into ADP1 cells. CbtA stops cell growth, which could yield in a better WE accumulation. Other cell growth stopping methods were used as well to test this hypothesis. The cell growth was stopped in two different tests with antibiotics and by changing the carbon source.
Arabinose induction did not affect the transformed cells, as their cell growth rate remain unchanged compared to the controls. Thus the effect of cbtA on WE accumulation on n-rich conditions could not be evaluated. However, additional tests showed that stopping the cell growth could yield in better WE levels in n-rich conditions.
The purpose of the thesis was to study whether ADP1 can genetically modified to produce WEs more efficiently in nitrogen-rich conditions. In order to achieve more efficient production, a plasmid with a gene for a cytoskeleton-binding toxin (cbtA) from Escherchia coli and with an arabinose inducible promoter was constructed and then transformed into ADP1 cells. CbtA stops cell growth, which could yield in a better WE accumulation. Other cell growth stopping methods were used as well to test this hypothesis. The cell growth was stopped in two different tests with antibiotics and by changing the carbon source.
Arabinose induction did not affect the transformed cells, as their cell growth rate remain unchanged compared to the controls. Thus the effect of cbtA on WE accumulation on n-rich conditions could not be evaluated. However, additional tests showed that stopping the cell growth could yield in better WE levels in n-rich conditions.