Biofunctionalization of zirconia based materials by immobilization of alp in tissue engineering applications
Ezazi, Nazanin Zanjanizadeh (2014)
2014
Master's Degree Programme in Biomedical Engineering
Tieto- ja sähkötekniikan tiedekunta - Faculty of Computing and Electrical Engineering
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Hyväksymispäivämäärä
2014-12-03
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tty-201412051606
https://urn.fi/URN:NBN:fi:tty-201412051606
Tiivistelmä
The aim of this project is to modify Zirconia surface to perform as a bioactive material in contact with biological tissue with high stability and activity. Alumina was also modified in order to compare the properties with Zirconia in the same laboratory condition. The protein used in this test is alkaline phosphatase (ALP) which improves mineralization and creation of hydroxyapatite in bone formation process. Adsorption and covalent bonding immobilization were investigated on both ceramics. The modified ceramic surfaces also tested in contact with and without human body cells, in biological condition in vitro.
This project contains laboratory tests for powders and planar surfaces. Characterizations of zirconia powder, such as IEP (isoelectric point) were investigated after ALP immobilization. In addition, ALP functionalized planar surface and its behaviour was studied in vitro in simulated body conditions with and without presence of osteoblast-like cells.
Main results of these studies supported that high mineralization accomplished by ALP functionalized zirconia in compare with non-functionalized zirconia in in vitro. In summary, the results of this research indicate the successful immobilization and surface modification of zirconia.
In addition, zirconia powder silanized by APTES, as the initial step of surface modification, showed acceptable stability in room temperature during 28 days.
In case of comparison between physical adsorption and covalent bonding methods, it can be observed that physical adsorption represents higher enzyme attachment. However, these attachments do not show suitable stability. In all the experiments regarding modification of the particle surface, Zirconia powder illustrates higher potential for protein (ALP) immobilization compare to Alumina.
This project contains laboratory tests for powders and planar surfaces. Characterizations of zirconia powder, such as IEP (isoelectric point) were investigated after ALP immobilization. In addition, ALP functionalized planar surface and its behaviour was studied in vitro in simulated body conditions with and without presence of osteoblast-like cells.
Main results of these studies supported that high mineralization accomplished by ALP functionalized zirconia in compare with non-functionalized zirconia in in vitro. In summary, the results of this research indicate the successful immobilization and surface modification of zirconia.
In addition, zirconia powder silanized by APTES, as the initial step of surface modification, showed acceptable stability in room temperature during 28 days.
In case of comparison between physical adsorption and covalent bonding methods, it can be observed that physical adsorption represents higher enzyme attachment. However, these attachments do not show suitable stability. In all the experiments regarding modification of the particle surface, Zirconia powder illustrates higher potential for protein (ALP) immobilization compare to Alumina.