Electrical impedance tomography applied to stem cells in hydrogel scaffold
Lehti-Polojärvi, Mari (2014)
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Electrical impedance tomography (EIT) could provide a label free, non-invasive and fast method for cell culture monitoring. This thesis is a feasibility study on what kind of results EIT may provide when cells are cultured in a 3D hydrogel scaffold. The work is divided into two parts: (1) electrical impedance spectroscopy (EIS) measurements and (2) computer modelling of the EIT setup. In the EIS study part, gellan gum (GG) hydrogels and GG hydrogels with encapsulated stem cells are measured using the impedance spectroscope HF2IS device (Zurich Instruments AG, Switzerland). In the second study part, two dimensional EIT computer models are done in COMSOL Multiphysics. The sensitivity field of EIT setup is simulated in order to find the optimal electrode locations and resistivity values for the aqueous solution used between electrodes and the sample. The EIS experiments indicate that the impedance value is dependent on the amount and viability of cells. Optimal electrode configuration and resistivity values are obtained by the EIT model and these parameters can be used in the future EIT measurements.