Hyppää sisältöön
    • Suomeksi
    • In English
Trepo
  • Suomeksi
  • In English
  • Kirjaudu
Näytä viite 
  •   Etusivu
  • Trepo
  • Opinnäytteet - ylempi korkeakoulututkinto
  • Näytä viite
  •   Etusivu
  • Trepo
  • Opinnäytteet - ylempi korkeakoulututkinto
  • Näytä viite
JavaScript is disabled for your browser. Some features of this site may not work without it.

Developing synthetic biology tools for lignocellulose degradation

Munawar, Mustafa Ahmad Jr (2014)

 
Avaa tiedosto
Munawar.pdf (2.438Mt)
Lataukset: 



Munawar, Mustafa Ahmad Jr
2014

Master's Degree Programme in Science and Bioengineering
Luonnontieteiden tiedekunta - Faculty of Natural Sciences
This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.
Hyväksymispäivämäärä
2014-11-05
Näytä kaikki kuvailutiedot
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tty-201410301533
Tiivistelmä
Lignocellulose is mainly composed of cellulose, hemicelluloses and lignin. Lignocellulose is abundant in nature and hence an attractive low cost feedstock for bioprocesses such as biofuel production. Bioproduction initially requires separation and hydrolysis of polysaccharides content of lignocellulose. Lignocellulose is decomposed either by physiochemical means or by biodegradation. Biodegradation is accomplished by fungi and bacteria in nature.
Unfortunately production of biofuels from lignocellulose is an expensive process. To increase the economic feasibility of the process, various aspects have been focused including the biological means of degradation. In the current project, KillerRed (KR) protein was engineered and tested for its capability to degrade cellulose. The KR protein was added with a cellulose binding peptide (CBP) at its N terminal. The engineered protein CBP-KR was expressed in Escherichia coli cells using conventional expression system and purified.
The binding property of purified CBP-KR was analyzed by simply incubating the protein solution with different cellulosic substrates and observing changes in fluorescence signals after incubations. The degradation capability of CBP-KR was tested by a modified Congo red assay. The CBP-KR exhibited clear binding to printing paper but degradation of carboxymethyl cellulose (CMC) could not be recorded by the modified Congo red assay.
Kokoelmat
  • Opinnäytteet - ylempi korkeakoulututkinto [40600]
Kalevantie 5
PL 617
33014 Tampereen yliopisto
oa[@]tuni.fi | Tietosuoja | Saavutettavuusseloste
 

 

Selaa kokoelmaa

TekijätNimekkeetTiedekunta (2019 -)Tiedekunta (- 2018)Tutkinto-ohjelmat ja opintosuunnatAvainsanatJulkaisuajatKokoelmat

Omat tiedot

Kirjaudu sisäänRekisteröidy
Kalevantie 5
PL 617
33014 Tampereen yliopisto
oa[@]tuni.fi | Tietosuoja | Saavutettavuusseloste