Development of 3D bioprinting inks based on tandem crosslinked hydrogels
Kesti, Matti (2013)
2013
Materiaalitekniikan koulutusohjelma
Teknisten tieteiden tiedekunta - Faculty of Engineering Sciences
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Hyväksymispäivämäärä
2013-10-09
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tty-201310211357
https://urn.fi/URN:NBN:fi:tty-201310211357
Tiivistelmä
In the future the field of medicine will be directed towards tailored treatments and per-sonalized medicine. In personalized medicine the implant is designed for the patient’s needs in terms of the shape of the implant and the cells encapsulated into the implant, which are harvested from the patient itself. By these means, the produced implant is mimicking the designated tissue and the cells are fully biocompatible with the surround-ing tissue. By utilizing rapid prototyping in biomaterial scaffold or tissue graft production, the patient optimized implants could be produced. One promising rapid prototyping technique is bioprinting.
The aim of this thesis was to investigate different material combinations to be used as bioinks for cartilage production. In this thesis, three cartilage specific tandem cross-linked hydrogel bioinks were created and analyzed. Furthermore, different constructs and printed composite structures were created to prove the printability and resolution of these bioinks. Materials used in this study were hyaluronic acid, chondroitin sulfate and dextran which were grafted with methacrylate groups. These materials were mixed with hyaluronic acid grafted with poly(N-isopropylacrylamine) (HA-PNIPAM).
The two-component hydrogels used were novel in regards to utilize HA-PNIPAM in 3D bioprinting and in using tandem crosslinking combining thermo and photosensitive crosslinking in the printing. By combining the two crosslinking methods we were able to create three different bioinks mimicking the cartilage layered structures. Tandem crosslinked bioinks kept the resolution of the printing process and they had an increased modulus after UV curing. The implants produced had insufficient mechanical properties compared to native cartilage, however, the printability and the resolutions were on suf-ficient levels. Further development to control the temperature gelation is required and the viability assays for the bioinks should be investigated.
The aim of this thesis was to investigate different material combinations to be used as bioinks for cartilage production. In this thesis, three cartilage specific tandem cross-linked hydrogel bioinks were created and analyzed. Furthermore, different constructs and printed composite structures were created to prove the printability and resolution of these bioinks. Materials used in this study were hyaluronic acid, chondroitin sulfate and dextran which were grafted with methacrylate groups. These materials were mixed with hyaluronic acid grafted with poly(N-isopropylacrylamine) (HA-PNIPAM).
The two-component hydrogels used were novel in regards to utilize HA-PNIPAM in 3D bioprinting and in using tandem crosslinking combining thermo and photosensitive crosslinking in the printing. By combining the two crosslinking methods we were able to create three different bioinks mimicking the cartilage layered structures. Tandem crosslinked bioinks kept the resolution of the printing process and they had an increased modulus after UV curing. The implants produced had insufficient mechanical properties compared to native cartilage, however, the printability and the resolutions were on suf-ficient levels. Further development to control the temperature gelation is required and the viability assays for the bioinks should be investigated.