Insulin resistance is associated with altered amino acid metabolism and adipose tissue dysfunction in normoglycemic women
Wiklund, Petri; Zhang, Xiaobo; Pekkala, Satu; Autio, Reija; Kong, Lingjia; Yang, Yifan; Keinänen-Kiukaanniemi, Sirkka; Alen, Markku; Cheng, Sulin (2016)
Wiklund, Petri
Zhang, Xiaobo
Pekkala, Satu
Autio, Reija
Kong, Lingjia
Yang, Yifan
Keinänen-Kiukaanniemi, Sirkka
Alen, Markku
Cheng, Sulin
2016
Scientific Reports 6
24540
Terveystieteiden yksikkö - School of Health Sciences
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:uta-201605191616
https://urn.fi/URN:NBN:fi:uta-201605191616
Tiivistelmä
Insulin resistance is associated adiposity, but the mechanisms are not fully understood. In this study, we aimed to identify early metabolic alterations associated with insulin resistance in normoglycemic women with varying degree of adiposity. One-hundred and ten young and middle-aged women were divided into low and high IR groups based on their median HOMA-IR (0.9 ± 0.4 vs. 2.8 ± 1.2). Body composition was assessed using DXA, skeletal muscle and liver fat by proton magnetic resonance spectroscopy, serum metabolites by nuclear magnetic resonance spectroscopy and adipose tissue and skeletal muscle gene expression by microarrays. High HOMA-IR subjects had higher serum branched-chain amino acid concentrations (BCAA) (p < 0.05 for both). Gene expression analysis of subcutaneous adipose tissue revealed significant down-regulation of genes related to BCAA catabolism and mitochondrial energy metabolism and up-regulation of several inflammation-related pathways in high HOMA-IR subjects (p < 0.05 for all), but no differentially expressed genes in skeletal muscle were found. In conclusion, in normoglycemic women insulin resistance was associated with increased serum BCAA concentrations, down-regulation of mitochondrial energy metabolism and increased expression of inflammation-related genes in the adipose tissue.
Kokoelmat
- Artikkelit [6140]