Deep vascular imaging in wounds by two-photon fluorescence microscopy
Yanez, Ciceron; Morales, Alma; Yue, Xiling; Urakami, Takeo; Komatsu, Masanobu; Järvinen, Tero AH; Belfield, Kevin (2013)
Yanez, Ciceron
Morales, Alma
Yue, Xiling
Urakami, Takeo
Komatsu, Masanobu
Järvinen, Tero AH
Belfield, Kevin
2013
Plos ONE 8 7
1-6
Lääketieteen yksikkö - School of Medicine
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Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:uta-201311191603
https://urn.fi/URN:NBN:fi:uta-201311191603
Kuvaus
Public Library of Science open access
Tiivistelmä
Deep imaging within tissue (over 300 μm) at micrometer resolution has become possible with the advent of two-photon fluorescence microscopy (2PFM). The advantages of 2PFM have been used to interrogate endogenous and exogenous fluorophores in the skin. Herein, we employed the integrin (cell-adhesion proteins expressed by invading angiogenic blood vessels) targeting characteristics of a two-photon absorbing fluorescent probe to image new vasculature and fibroblasts up to ≈ 1600 μm within wound (neodermis)/granulation tissue in lesions made on the skin of mice. Reconstruction revealed three dimensional (3D) architecture of the vascular plexus forming at the regenerating wound tissue and the presence of a fibroblast bed surrounding the capillaries. Biologically crucial events, such as angiogenesis for wound healing, may be illustrated and analyzed in 3D on the whole organ level, providing novel tools for biomedical applications.
Kokoelmat
- Artikkelit [6139]