Genome wide scan for prostate cancer susceptibility genes
HA, NA TI (2008)
2008
Bioinformatiikka - Bioinformatics
Lääketieteellinen tiedekunta - Faculty of Medicine
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Hyväksymispäivämäärä
2008-08-18
Julkaisun pysyvä osoite on
https://urn.fi/urn:nbn:fi:uta-1-19312
https://urn.fi/urn:nbn:fi:uta-1-19312
Tiivistelmä
Background and aims: Prostate cancer is the leading cancer type in Finnish men. It was suggested that 5% to 10% of incident cases are attributed to rare, highly penetrated alleles in single gene forms of disease. The aim of the study was to use genome wide linkage scan in 56 Finnish Families with multiple prostate cancer cases to detect possible prostate cancer susceptibility genes.
Methods: Genotyping data of 490 microsatellite markers was analyzed with two point, and multipoint parametric and non parametric linkage analyses. Using Prostate cancer genotype data as input, Mendelian errors were checked by Pedcheck program. Two Point linkage analyses were carried out by FastLink program. Singlepoint nonparametric,multipoint parametric and nonparametric analyses were carried out by GENEHUNTER program.
Results: The most significant results are obtained from chromosome 13 which gave the best two point LOD score 2.67 with marker D13S173 at 13q33, chromosome 17 at 17q21, where the best two point LOD score was 2.46, The third significant LOD score come from chromosome 3 at 3q26, where the best two point LOD score was 2.38 (theta=0.1) with marker D3S1565, and chromosome X at Xq27, where the best two point LOD score 2.03 (theta =0.2) with marker DXS1227.
Conclusion: Results from chromosome 3, 8, 12, and X, support previous linkage analyses. In addition, the present linkage analyses also reveal areas which are not presented in the previous linkage analyses, such as chromosome 17 at 17q21, chromosome 13 at 13q33, chromosome 2 at 2q37 and chromosome 6 at 6p21.
Methods: Genotyping data of 490 microsatellite markers was analyzed with two point, and multipoint parametric and non parametric linkage analyses. Using Prostate cancer genotype data as input, Mendelian errors were checked by Pedcheck program. Two Point linkage analyses were carried out by FastLink program. Singlepoint nonparametric,multipoint parametric and nonparametric analyses were carried out by GENEHUNTER program.
Results: The most significant results are obtained from chromosome 13 which gave the best two point LOD score 2.67 with marker D13S173 at 13q33, chromosome 17 at 17q21, where the best two point LOD score was 2.46, The third significant LOD score come from chromosome 3 at 3q26, where the best two point LOD score was 2.38 (theta=0.1) with marker D3S1565, and chromosome X at Xq27, where the best two point LOD score 2.03 (theta =0.2) with marker DXS1227.
Conclusion: Results from chromosome 3, 8, 12, and X, support previous linkage analyses. In addition, the present linkage analyses also reveal areas which are not presented in the previous linkage analyses, such as chromosome 17 at 17q21, chromosome 13 at 13q33, chromosome 2 at 2q37 and chromosome 6 at 6p21.