Influence of Hsp90 and HDAC Inhibition and Tubulin Acetylation on Perinuclear Protein Aggregation in Human Retinal Pigment Epithelial Cells
Ryhänen, Tuomas; Viiri, Johanna; Hyttinen, Juha M.T; Uusitalo, Hannu; Salminen, Antero; Kaarniranta, Kai (2011)
Ryhänen, Tuomas
Viiri, Johanna
Hyttinen, Juha M.T
Uusitalo, Hannu
Salminen, Antero
Kaarniranta, Kai
2011
Journal of Biomedicine and Biotechnology 2011
798052
Lääketieteen yksikkö - School of Medicine
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Julkaisun pysyvä osoite on
https://urn.fi/urn:nbn:uta-3-707
https://urn.fi/urn:nbn:uta-3-707
Kuvaus
Hindawi open access
Tiivistelmä
Retinal pigment epithelial (RPE) cells are continually exposed to oxidative stress that contributes to protein misfolding, aggregation and functional abnormalities during aging. The protein aggregates formed at the cell periphery are delivered along the microtubulus network by dynein-dependent retrograde trafficking to a juxtanuclear location. We demonstrate that Hsp90 inhibition by geldanamycin can effectively suppress proteasome inhibitor, MG-132-induced protein aggregation in a way that is independent of HDAC inhibition or the tubulin acetylation levels in ARPE-19 cells. However, the tubulin acetylation and polymerization state affects the localization of the proteasome-inhibitor-induced aggregation. These findings open new perspectives for understanding the pathogenesis of protein aggregation in retinal cells and can be useful for the development of therapeutic treatments to prevent retinal cell deterioration.
Kokoelmat
- Artikkelit [6139]