Photoactivated Nitric Oxide Release from SNAP-Loaded Bioactive Glass Scaffolds incorporated with Persistent luminescent particles
Kumar, Sathiya (2026)
2026
Master's Programme in Biomedical Sciences and Engineering
Lääketieteen ja terveysteknologian tiedekunta - Faculty of Medicine and Health Technology
Hyväksymispäivämäärä
2026-07-29
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202607158357
https://urn.fi/URN:NBN:fi:tuni-202607158357
Tiivistelmä
Antimicrobial resistance has emerged as one of the biggest threats to health in the 21st century across the globe, necessitating the need for antibiotic-free therapeutic approaches. Nitric Oxide (NO) releasing compounds have come to light as a potential alternative, providing broad-spectrum antimicrobial activity with a minimal risk of contributing to antimicrobial resistance. The aim of this project is to investigate the release of nitric oxide from a multifunctional theranostic bio composite consisting of 1393B20 bioactive glass, S-nitroso-N-acetyl-DL-penicillamine (SNAP), and persistent luminescent particles (PeL) to offer a targeted, antibiotic-free antimicrobial approach to overcome the limitations of antimicrobial resistance.
The objective was to assess the photostability of SNAP in Tris buffer at different concentrations under different light (white light, UV irradiation, and dark conditions) and experimental conditions (pulsed vs continuous illumination, in static immersion and semi-dynamic) using UV-Vis spectrophotometry; to examine the effect of persistent luminescent particles integrated into 1393B20 bioactive glass on SNAP stability under similar conditions; to analyse the SNAP degradation byproducts; to quantify the release of NO using NO Analyser; to determine the antioxidant capacity of NO; and to evaluate the cytocompatibility of the system through cell viability analysis.
The findings indicated that exposing SNAP to a light source effectively triggered its degradation and led to the release of NO, whereas SNAP exhibited optimal stability under dark conditions, highlighting the significance of light as a trigger for controlled NO release thereby validating its light-activated NO release capability. Quantification of NO using NO Analyser (NOA) revealed that release of NO was consistent with the activation of the light source. Antioxidant Assay showed that SNAP solutions have antioxidant capacity, with higher concentrations having higher total antioxidative capacity. Live and Dead assay revealed that cells exposed to SNAP under white light showed improved cell viability when compared to the cells exposed to SNAP under dark conditions.
The objective was to assess the photostability of SNAP in Tris buffer at different concentrations under different light (white light, UV irradiation, and dark conditions) and experimental conditions (pulsed vs continuous illumination, in static immersion and semi-dynamic) using UV-Vis spectrophotometry; to examine the effect of persistent luminescent particles integrated into 1393B20 bioactive glass on SNAP stability under similar conditions; to analyse the SNAP degradation byproducts; to quantify the release of NO using NO Analyser; to determine the antioxidant capacity of NO; and to evaluate the cytocompatibility of the system through cell viability analysis.
The findings indicated that exposing SNAP to a light source effectively triggered its degradation and led to the release of NO, whereas SNAP exhibited optimal stability under dark conditions, highlighting the significance of light as a trigger for controlled NO release thereby validating its light-activated NO release capability. Quantification of NO using NO Analyser (NOA) revealed that release of NO was consistent with the activation of the light source. Antioxidant Assay showed that SNAP solutions have antioxidant capacity, with higher concentrations having higher total antioxidative capacity. Live and Dead assay revealed that cells exposed to SNAP under white light showed improved cell viability when compared to the cells exposed to SNAP under dark conditions.