Functionalized cellulose nanocrystals reinforced PLA-gelatin electrospun fibers for potential antibacterial wound dressing and coating applications

Abstract

This study addresses the critical need for effective antibacterial materials by exploring the innovative integration of dimethyloctadecyl [3-(trimethoxysilyl)propyl] ammonium chloride (DTSACl) onto cellulose nanocrystal (CNC), followed by its incorporation into polylactic acid and gelatin matrices to engineer antibacterial nanofiber mats. The modification of CNC with DTSACl (QACNC) was studied and confirmed by FT-IR, 13C NMR, and XRD analysis. Furthermore, the impact of such addition on the morphology, mechanical, hydrophobic properties, and antibacterial efficacy of the resultant QACNC nanofibers were thoroughly investigated. It was found that the QACNC inhibited the growth of Staphylococcus aureus by 99 % but had no effect on Pseudomonas aeruginosa at 125 μg/mL concentration. Various concentrations of QACNC were blended into the as-spun PLA/Gel solutions before spinning or coated onto spun PLA/Gel nanofiber mats. There was a minor antibacterial effect observed with PLA/Gel mats blended with up to 3 wt% QCNC, while the average inhibition for PLA/Gel/QACNC 5 wt% was 68.3 % ± 36.5 %. By increasing the amount of QACNC blended into the polymer matrix, the human dermal fibroblast (HDF) cell viability decreased, indicating that optimizing QACNC concentrations is crucial for maintaining cell viability while ensuring effective antibacterial performance. Given the enhanced antibacterial properties, the fabricated textiles hold significant potential for applications in medical textiles and wound dressings.