Pulmonary toxicity of Fe₂O₃, ZnFe₂O₄, NiFe₂O₄ and NiZnFe₄O₈ nanomaterials: Inflammation and DNA strand breaks

Abstract

<p>Exposure to metal oxide nanomaterials potentially occurs at the workplace. We investigated the toxicity of two Fe-oxides: Fe₂O₃ nanoparticles and nanorods; and three MFe₂O₄ spinels: NiZnFe₄O₈, ZnFe₂O₄, and NiFe₂O₄ nanoparticles. Mice were dosed 14, 43 or 128 μg by intratracheal instillation. Recovery periods were 1, 3, or 28 days. Inflammation – neutrophil influx into bronchoalveolar lavage (BAL) fluid – occurred for Fe₂O₃ rods (1 day), ZnFe₂O₄ (1, 3 days), NiFe₂O₄ (1, 3, 28 days), Fe₂O₃ (28 days) and NiZnFe₄O₈ (28 days). Conversion of mass-dose into specific surface-area-dose showed that inflammation correlated with deposited surface area and consequently, all these nanomaterials belong to the so-called low-solubility, low-toxicity class. Increased levels of DNA strand breaks were observed for both Fe₂O₃ particles and rods, in BAL cells three days post-exposure. To our knowledge, this is, besides magnetite (Fe₃O₄), the first study of the pulmonary toxicity of MFe₂O₄ spinel nanomaterials.</p>