Comparison of Scenedesmus acuminatus and Chlorella vulgaris cultivation in liquid digestates from anaerobic digestion of pulp and paper industry and municipal wastewater treatment sludge

Abstract

Two microalgae, Chlorella vulgaris and Scenedesmus acuminatus, were batch cultivated separately in two types of diluted liquid digestates. The first digestate (ADPP) was obtained from a mesophilic laboratory digester treating biosludge from a pulp and paper industry wastewater treatment plant. The second digestate (ADMW) was collected from a full-scale mesophilic anaerobic digester treating-mixed municipal wastewater treatment sludge. The highest biomass production (as volatile suspended solids, VSS), 8.2–9.4 g L−1, was obtained with S. acuminatus in ADPP. C. vulgaris in ADMW had the lowest biomass production, reaching 2.0 g L−1. Both microalgae removed ammonium efficiently from ADPP (99.9% removal) while the final ammonium removal efficiencies from ADMW with S. acuminatus and C. vulgaris were only 44.0 and 23.8%, respectively. The phosphate removal efficiencies from both ADPP and ADMW were higher than 96.9% with both microalgae. The highest carbohydrate content (60.5%) was obtained with S. acuminatus cultivated in ADPP. Scenedesmus acuminatus in ADPP showed one of the highest biomass production yields that have been reported for microalgae in real wastewater-derived nutrient sources. Consequently, this combination is promising for developing biorefinery and biofuel applications in the pulp and paper industry.