Gallol-tethered hyaluronic acid reduced/stabilized synthesis of gold nanoparticles
Lignell, Veera (2024)
2024
Bioteknologian ja biolääketieteen tekniikan kandidaattiohjelma - Bachelor's Programme in Biotechnology and Biomedical Engineering
Lääketieteen ja terveysteknologian tiedekunta - Faculty of Medicine and Health Technology
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Hyväksymispäivämäärä
2024-05-14
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202404254607
https://urn.fi/URN:NBN:fi:tuni-202404254607
Tiivistelmä
The purpose of this theses was to research if gallic acid functionalized hyaluronic acid (HA-GA) will be able to coat gold nanoparticles along with reducing and stabilizing the nanoparticle. If the coating material will act as a reducing and stabilizing agent the synthesis can be called a green synthesis. Green synthesis is defined as if there is no additional reagent to take care of the reduction. Hyaluronic acid and gold nanoparticles are widely used in biomedical application because they have good biocompatibility. Hyaluronic acid is good especially for its water binding characteristics and that the molecule has a negative charge, so it works good with cellular membranes. Gold is the most used metal for nanoparticles and on top of good biocompatibility it is non-immunogenic and gold nanoparticles stability increases in biological environments.
First HA-GA was synthesized from hyaluronic acid and galloyl hydrazide with EDC coupling reaction. The reaction uses carboxylic acid that react with amine to amides. From the synthesized HA-GA nuclear magnetic resonance (NMR) was measured to confirm that the synthesis was successful. The NMR results from HA-GA were compared with NMR from HA and from those it could be confirmed that HA-GA was synthesized successfully.
Second gold nanoparticle was synthesized using HA-GA as a coating as well as a stabilizing and reducing agent. From the synthesized gold nanoparticles, size distribution and zeta potential were measured. Scanning electron microscopy and transmission electron microscopy images were also taken from the particle. Final size of the particle was determined from the scanning electron microscopy image, and the final size was 70-80 nanometres. Zeta potential of the particle was -36 mV. Overall, the particle size was evaluated to be feasible and the strong negative zeta potential suggests good interaction with cell membranes. Coated AuNPs are promising as a material and they have several possible applications that are still unknown. In the future function of the particles could be studied in hybrid hydrogels and targeted drug delivery applications.
First HA-GA was synthesized from hyaluronic acid and galloyl hydrazide with EDC coupling reaction. The reaction uses carboxylic acid that react with amine to amides. From the synthesized HA-GA nuclear magnetic resonance (NMR) was measured to confirm that the synthesis was successful. The NMR results from HA-GA were compared with NMR from HA and from those it could be confirmed that HA-GA was synthesized successfully.
Second gold nanoparticle was synthesized using HA-GA as a coating as well as a stabilizing and reducing agent. From the synthesized gold nanoparticles, size distribution and zeta potential were measured. Scanning electron microscopy and transmission electron microscopy images were also taken from the particle. Final size of the particle was determined from the scanning electron microscopy image, and the final size was 70-80 nanometres. Zeta potential of the particle was -36 mV. Overall, the particle size was evaluated to be feasible and the strong negative zeta potential suggests good interaction with cell membranes. Coated AuNPs are promising as a material and they have several possible applications that are still unknown. In the future function of the particles could be studied in hybrid hydrogels and targeted drug delivery applications.
Kokoelmat
- Kandidaatintutkielmat [10220]