Electrical Stimulation Platform for Cells
Biswas, Sabya Sachi (2023)
2023
Bioteknologian ja biolääketieteen tekniikan maisteriohjelma - Master's Programme in Biotechnology and Biomedical Engineering
Lääketieteen ja terveysteknologian tiedekunta - Faculty of Medicine and Health Technology
This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.
Hyväksymispäivämäärä
2023-12-21
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-2023121310779
https://urn.fi/URN:NBN:fi:tuni-2023121310779
Tiivistelmä
Electrical Stimulation (ES) has been shown to increase the functional properties of cells. In particular, with the cardiomyocytes that are cultured in vitro, pulsed electrical stimulation has been shown to induce a variety of responses including migration, proliferation, differentiation, and gene expression. However, commercial electrical stimulators are costly and complex devices. Customized solutions often have limited parameter tunability, less compatibility as well as less feedback control. These challenges are still preventing the electrical stimulation to cells to reach its full potential and limits the study of different ES protocols.
In order to take step towards to achieve maximum potential of the electrical stimulation, a multipurpose compact electrical stimulation device was designed and developed in this thesis for delivering controlled ES to cardiomyocytes (CMs) at an affordable cost. The ES device is based on open-source programmable microcontroller named Arduino and is connected to a custom designed circuit board. Arduino was accessible and was programmed based on a potential user need. Furthermore, the circuit board contained components including multiplexer and operational amplifier which amplify the signal based on Arduino input. To verify and characterize the device, the setup was simultaneously tested with different ES parameter patterns on NI USB measurement card. Based on the studied literature, parameter ranges were selected and tested on the device. Monophasic square ES pulse was used for the test.
From the characterization test, it was confirmed that the ES parameters provided by the instrument were accurate, compliant, and reliable. The device was tested with eight different parameters arrangement where four arrangements with minimum 1 V amplitude and other four arrangement with maximum 9 V amplitude were used. The duration of the measurement of each parameter arrangement was selected randomly so that minimum of 10 pulses were recorded in a single measurement. From the results, it was found that all the parameters including frequency and pulse length were providing the same output value (with 0.01 value difference) that was given as input. The output pulse delay was higher (smaller or equal to 1 %) than the given input delay which indicate that the device takes longer time than the given input delay while creating pulses. In addition, there was not much noise observed in the pulse waveforms although amplitude 9 V arrangements had less noise (0.715 mV) than the amplitude 1 V arrangements (0.84 mV). The master thesis study indicates that, the ES device is functional and can be used for the stimulation of cardiomyocytes, but further improvements are needed to make the device work more perfectly.
In order to take step towards to achieve maximum potential of the electrical stimulation, a multipurpose compact electrical stimulation device was designed and developed in this thesis for delivering controlled ES to cardiomyocytes (CMs) at an affordable cost. The ES device is based on open-source programmable microcontroller named Arduino and is connected to a custom designed circuit board. Arduino was accessible and was programmed based on a potential user need. Furthermore, the circuit board contained components including multiplexer and operational amplifier which amplify the signal based on Arduino input. To verify and characterize the device, the setup was simultaneously tested with different ES parameter patterns on NI USB measurement card. Based on the studied literature, parameter ranges were selected and tested on the device. Monophasic square ES pulse was used for the test.
From the characterization test, it was confirmed that the ES parameters provided by the instrument were accurate, compliant, and reliable. The device was tested with eight different parameters arrangement where four arrangements with minimum 1 V amplitude and other four arrangement with maximum 9 V amplitude were used. The duration of the measurement of each parameter arrangement was selected randomly so that minimum of 10 pulses were recorded in a single measurement. From the results, it was found that all the parameters including frequency and pulse length were providing the same output value (with 0.01 value difference) that was given as input. The output pulse delay was higher (smaller or equal to 1 %) than the given input delay which indicate that the device takes longer time than the given input delay while creating pulses. In addition, there was not much noise observed in the pulse waveforms although amplitude 9 V arrangements had less noise (0.715 mV) than the amplitude 1 V arrangements (0.84 mV). The master thesis study indicates that, the ES device is functional and can be used for the stimulation of cardiomyocytes, but further improvements are needed to make the device work more perfectly.