Comparative analysis of two paradigm bacteriophytochromes reveals opposite functionalities in two-component signaling
Multamäki, Elina; Nanekar, Rahul; Morozov, Dmitry; Lievonen, Topias; Golonka, David; Wahlgren, Weixiao Yuan; Stucki-Buchli, Brigitte; Rossi, Jari; Hytönen, Vesa P.; Westenhoff, Sebastian; Ihalainen, Janne A.; Möglich, Andreas; Takala, Heikki (2021-07)
Multamäki, Elina
Nanekar, Rahul
Morozov, Dmitry
Lievonen, Topias
Golonka, David
Wahlgren, Weixiao Yuan
Stucki-Buchli, Brigitte
Rossi, Jari
Hytönen, Vesa P.
Westenhoff, Sebastian
Ihalainen, Janne A.
Möglich, Andreas
Takala, Heikki
07 / 2021
4394
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202108116515
https://urn.fi/URN:NBN:fi:tuni-202108116515
Kuvaus
Peer reviewed
Tiivistelmä
Bacterial phytochrome photoreceptors usually belong to two-component signaling systems which transmit environmental stimuli to a response regulator through a histidine kinase domain. Phytochromes switch between red light-absorbing and far-red light-absorbing states. Despite exhibiting extensive structural responses during this transition, the model bacteriophytochrome from Deinococcus radiodurans (DrBphP) lacks detectable kinase activity. Here, we resolve this long-standing conundrum by comparatively analyzing the interactions and output activities of DrBphP and a bacteriophytochrome from Agrobacterium fabrum (Agp1). Whereas Agp1 acts as a conventional histidine kinase, we identify DrBphP as a light-sensitive phosphatase. While Agp1 binds its cognate response regulator only transiently, DrBphP does so strongly, which is rationalized at the structural level. Our data pinpoint two key residues affecting the balance between kinase and phosphatase activities, which immediately bears on photoreception and two-component signaling. The opposing output activities in two highly similar bacteriophytochromes suggest the use of light-controllable histidine kinases and phosphatases for optogenetics.
Kokoelmat
- TUNICRIS-julkaisut [16929]