Improving hydrogel properties for better cell survival for transplantation
Samal, Jay Rabindra Kumar (2020)
2020
Degree Programme in Biomedical Sciences and Engineering, MSc (Tech)
Lääketieteen ja terveysteknologian tiedekunta - Faculty of Medicine and Health Technology
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Hyväksymispäivämäärä
2020-04-28
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202003302965
https://urn.fi/URN:NBN:fi:tuni-202003302965
Tiivistelmä
The free radical formation and resulting oxidative stress formed during cell transplantation can cause cellular damage, leading to prolonged healing times and reduction in efficacy of the implant. Depending on the site of transplantation and its distance from vasculature, cells may experience low oxygen concentrations, which can affect the proliferation and differentiation potential.
To improve cell survival and cellular behavior for transplantation, gallic acid (GA) incorporated hyaluronic acid hydrogel (HA-GA) was formulated and its properties were compared to hyaluronic acid (HA) hydrogels without gallic acid (HA-HA). Further, human mesenchymal stem cells (hMSCs) were cultured under hypoxia to observe effect of hypoxia on osteogenic differentiation.
The results obtained during this study suggest that HA-GA gels showed improved adhesive and antioxidant properties as compared to HA-HA gels. Both gels showed high elastic property during rheological studies and could maintain viability of hMSCs for 21 days of culture. Moreover, HA-GA gels also possessed immunomodulatory properties, and might reduce inflammation and damaging immune response at the transplantation site. Thus, the incorporation of gallic acid moieties into HA gels could be a viable strategy to combat oxidative stress encountered during transplantation procedures, while improving adhesiveness to the target tissue, thus reducing wound healing time and promoting better quality of healing. This may especially be useful for individuals with reduced wound healing capacity, for example, diabetic individuals. Hydrogels can increase the osteogenic differentiation potential of hMSCs and as such could be applied as transplants for treating bony injury. Furthermore, the application of hypoxic conditions to MSC cell culture may improve cell viability and cell number upon transplantation, which may help to overcome limitations of low cell numbers currently restricting tissue engineering applications and promote understanding and development of functional tissue constructs.
En masse, the results obtained during the study could lead towards improvement of cellular behavior and survival in hydrogels for cell transplantation
To improve cell survival and cellular behavior for transplantation, gallic acid (GA) incorporated hyaluronic acid hydrogel (HA-GA) was formulated and its properties were compared to hyaluronic acid (HA) hydrogels without gallic acid (HA-HA). Further, human mesenchymal stem cells (hMSCs) were cultured under hypoxia to observe effect of hypoxia on osteogenic differentiation.
The results obtained during this study suggest that HA-GA gels showed improved adhesive and antioxidant properties as compared to HA-HA gels. Both gels showed high elastic property during rheological studies and could maintain viability of hMSCs for 21 days of culture. Moreover, HA-GA gels also possessed immunomodulatory properties, and might reduce inflammation and damaging immune response at the transplantation site. Thus, the incorporation of gallic acid moieties into HA gels could be a viable strategy to combat oxidative stress encountered during transplantation procedures, while improving adhesiveness to the target tissue, thus reducing wound healing time and promoting better quality of healing. This may especially be useful for individuals with reduced wound healing capacity, for example, diabetic individuals. Hydrogels can increase the osteogenic differentiation potential of hMSCs and as such could be applied as transplants for treating bony injury. Furthermore, the application of hypoxic conditions to MSC cell culture may improve cell viability and cell number upon transplantation, which may help to overcome limitations of low cell numbers currently restricting tissue engineering applications and promote understanding and development of functional tissue constructs.
En masse, the results obtained during the study could lead towards improvement of cellular behavior and survival in hydrogels for cell transplantation