Production of alpha-olefin in Acinetobacter baylyi ADP1
Liu, Changshuo (2019)
2019
Biotekniikan DI-tutkinto-ohjelma - Degree Programme in Bioengineering
Lääketieteen ja terveysteknologian tiedekunta - Faculty of Medicine and Health Technology
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Hyväksymispäivämäärä
2019-12-04
Julkaisun pysyvä osoite on
http://urn.fi/URN:NBN:fi:tuni-201911286405
http://urn.fi/URN:NBN:fi:tuni-201911286405
Tiivistelmä
As energy and environmental issues become more and more serious, bioproduction of valuable chemicals, e.g. medium-chain alpha-olefins, as an alternative source become important increasingly. Medium-chain alpha-olefins, e.g. 1-undecene, are important hydrocarbon chemicals for various industrial applications. They can be used for industrial intermediates of detergents, cosmetic products, greases, lubricants, plasticizers, and “drop-in” next generation biofuel. Currently, medium-chain alpha-olefins are derived from fossil sources.
This study demonstrated Acinetobacter baylyi ADP1 as an excellent biological engineering platform for the bioproduction of medium-chain alpha-olefins. An iron-dependent desaturase/decarboxylase UndA and a membrane-bound desaturase-like enzyme UndB can convert fatty acids to corresponding olefins. Glyoxylate shunt blocking was applied for A. baylyi ADP1 to optimize biosynthesis of 1-undecene, the 1-undecene production titer of 350 ng/ml was obtained by the expression of UndA. Biosynthesis system based on UndB produced 331 ng/ml of 1-undecene. Expression of UndB is feasible in A. baylyi ADP1, which can make ADP1 to produce wider range of olefins (including olefins with even number of carbon atoms: C12 and C14). This study demonstrated the potential of UndB in A. baylyi ADP1 and a possible 1-undecene biosynthesis rate limiting factor. The bioproduction of 1-undecene in A. baylyi ADP1 should be improved and optimized in further researches.
This study demonstrated Acinetobacter baylyi ADP1 as an excellent biological engineering platform for the bioproduction of medium-chain alpha-olefins. An iron-dependent desaturase/decarboxylase UndA and a membrane-bound desaturase-like enzyme UndB can convert fatty acids to corresponding olefins. Glyoxylate shunt blocking was applied for A. baylyi ADP1 to optimize biosynthesis of 1-undecene, the 1-undecene production titer of 350 ng/ml was obtained by the expression of UndA. Biosynthesis system based on UndB produced 331 ng/ml of 1-undecene. Expression of UndB is feasible in A. baylyi ADP1, which can make ADP1 to produce wider range of olefins (including olefins with even number of carbon atoms: C12 and C14). This study demonstrated the potential of UndB in A. baylyi ADP1 and a possible 1-undecene biosynthesis rate limiting factor. The bioproduction of 1-undecene in A. baylyi ADP1 should be improved and optimized in further researches.