Performance testing and commercialization of regenerable biotinylated sensor slide
Saari, Sofia (2018)
2018
Bioteknologian tutkinto-ohjelma - Degree Programme in Biotechnology
Lääketieteen ja biotieteiden tiedekunta - Faculty of Medicine and Life Sciences
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Hyväksymispäivämäärä
2018-06-27
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:uta-201807092293
https://urn.fi/URN:NBN:fi:uta-201807092293
Tiivistelmä
Kinetics and binding measurements are often inevitable in biomedicine related research and industry. The highly sensitive and label free surface plasmon resonance (SPR) biosensor method has been used as an established method for biomolecular analysis. Multi-Parametric Surface Plasmon Resonance (MP-SPR) broadens the application range for interaction analyses and offers new insights for growing business markets. Enhanced regeneration capabilities would create significant competitive advantage possibilities for various applications. In addition, possibilities to gain revenues in the biosensor related markets have been growing. At the forefront of biotech and health related sectors, profitability is often made by combining data, analytics with technology and business.
In this thesis gold coated sensor slide surfaces were biotinylated and modified with thiol derivatives to examine the optimal functionality of sensors in terms of regeneration, storage circumstances and aging. The characteristics and quality of the modified surfaces were investigated with BioNavis multi parametric surface plasmon resonance (MP-SPR) device. Switchavidin was selected for injections because of its remarkable properties and ability to bind reversible to biotin. Immunoglobulin G (IgG) was chosen because the antibody is a relevant and widely used molecular tool. Biosensor related bio business aspects were studied in order to characterize market economy and understand universal needs to create business opportunities in order to grow revenues. Features related for attaining and maintaining competitiveness in biosensor related markets were studied. In addition, the results accomplished in this Master’s Thesis work, was aimed to gather additional information for BioNavis in order to enhance further business strategies. Biobusiness orientation was highlighted through the Thesis in order to advance translation of research results to industry and provide understanding about the relationships between science, technology, development and business.
Sensor slides regenerated surprisingly good and BioNavis can use the results as a part of the material in selling and marketing. The functional regeneration process and features for sensor slide’s optimal environment can be confirmed with the results. Aging and shelf life estimation needs further research in order to gain more specific results but provides an agenda for further research. Additionally, the kit could bring further value for the company. The results and observations made from the experiments provide useful data for the further research. Additionally, further optimization and studies can be made with results in order to innovate new business strategy interventions and build cross-sell services. This Master’s Thesis advocates further cooperation on biomedical science, technology and business among enterprises and academic sector.
In this thesis gold coated sensor slide surfaces were biotinylated and modified with thiol derivatives to examine the optimal functionality of sensors in terms of regeneration, storage circumstances and aging. The characteristics and quality of the modified surfaces were investigated with BioNavis multi parametric surface plasmon resonance (MP-SPR) device. Switchavidin was selected for injections because of its remarkable properties and ability to bind reversible to biotin. Immunoglobulin G (IgG) was chosen because the antibody is a relevant and widely used molecular tool. Biosensor related bio business aspects were studied in order to characterize market economy and understand universal needs to create business opportunities in order to grow revenues. Features related for attaining and maintaining competitiveness in biosensor related markets were studied. In addition, the results accomplished in this Master’s Thesis work, was aimed to gather additional information for BioNavis in order to enhance further business strategies. Biobusiness orientation was highlighted through the Thesis in order to advance translation of research results to industry and provide understanding about the relationships between science, technology, development and business.
Sensor slides regenerated surprisingly good and BioNavis can use the results as a part of the material in selling and marketing. The functional regeneration process and features for sensor slide’s optimal environment can be confirmed with the results. Aging and shelf life estimation needs further research in order to gain more specific results but provides an agenda for further research. Additionally, the kit could bring further value for the company. The results and observations made from the experiments provide useful data for the further research. Additionally, further optimization and studies can be made with results in order to innovate new business strategy interventions and build cross-sell services. This Master’s Thesis advocates further cooperation on biomedical science, technology and business among enterprises and academic sector.