Human embryonic stem cell -derived neural co-cultures
KAARELA, TIINA (2012)
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KAARELA, TIINA
2012
Biokemia - Biochemistry
Biolääketieteellisen teknologian yksikkö - Institute of Biomedical Technology
Hyväksymispäivämäärä
2012-06-01Tiivistelmä
ABSTRACT
Background and aims: Human embryonic stem cells are pluripotent stem cells with the potential to differentiate into any cell types of the human body. Differentiation of these stem cells into neuronal and glial cells enables the formation of different type of neural cell grafts. These grafts can be used in the treatment of different neurological disorders or injuries, such as spinal cord injuries. Traditionally neural cell transplantations have focused on the transplantation of only single cell types. In this study the aim was to form co-cultures of both neuronal and oligodendroglial cells, in order to mimic the in vivo myelination.
Methods: Human embryonic stem cells were differentiated into neurons and oligodendrocytes. Both of these cell populations were purified with fluorescence activated cell sorting and then cultured together. These co-cultures were aimed to form neural networks with myelinated axons. The gene and protein expression in co-cultures was studied.
Results: Maturation of oligodendrocyte precursors in co-cultures was detected, indicating that the cells were matured into myelinating oligodendrocytes.
Conclusions: Both neurons and oligodendrocytes did survive in the used co-culture conditions, and oligodendrocytes were able to mature into cells with myelinating capacity. Still, the design of co-culture formation and the myelin detection should be reconsidered in order to form successfully myelinating cultures. This study serves as a valuable base for the future optimization.
Asiasanat:Key words: oligodendrocyte, oligodendrocyte precursor, neural co-culture, human embryonic stem cell, fluorescence activated cell sorting
Background and aims: Human embryonic stem cells are pluripotent stem cells with the potential to differentiate into any cell types of the human body. Differentiation of these stem cells into neuronal and glial cells enables the formation of different type of neural cell grafts. These grafts can be used in the treatment of different neurological disorders or injuries, such as spinal cord injuries. Traditionally neural cell transplantations have focused on the transplantation of only single cell types. In this study the aim was to form co-cultures of both neuronal and oligodendroglial cells, in order to mimic the in vivo myelination.
Methods: Human embryonic stem cells were differentiated into neurons and oligodendrocytes. Both of these cell populations were purified with fluorescence activated cell sorting and then cultured together. These co-cultures were aimed to form neural networks with myelinated axons. The gene and protein expression in co-cultures was studied.
Results: Maturation of oligodendrocyte precursors in co-cultures was detected, indicating that the cells were matured into myelinating oligodendrocytes.
Conclusions: Both neurons and oligodendrocytes did survive in the used co-culture conditions, and oligodendrocytes were able to mature into cells with myelinating capacity. Still, the design of co-culture formation and the myelin detection should be reconsidered in order to form successfully myelinating cultures. This study serves as a valuable base for the future optimization.
Asiasanat:Key words: oligodendrocyte, oligodendrocyte precursor, neural co-culture, human embryonic stem cell, fluorescence activated cell sorting