A tube-source X-ray microtomography approach for quantitative 3D microscopy of optically challenging cell-cultured samples
Tamminen, Ilmari; Lehto, Kalle; Hannula, Markus; Ojansivu, Miina; Johansson, Laura; Kellomäki, Minna; Miettinen, Susanna; Aula, Antti; Ihalainen, Teemu; Hyttinen, Jari (2020)
Tamminen, Ilmari
Lehto, Kalle
Hannula, Markus
Ojansivu, Miina
Johansson, Laura
Kellomäki, Minna
Miettinen, Susanna
Aula, Antti
Ihalainen, Teemu
Hyttinen, Jari
2020
548
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202012199022
https://urn.fi/URN:NBN:fi:tuni-202012199022
Kuvaus
Peer reviewed
Tiivistelmä
Development and study of cell-cultured constructs, such as tissue-engineering scaffolds or organ-on-a-chip platforms require a comprehensive, representative view on the cells inside the used materials. However, common characteristics of biomedical materials, for example, in porous, fibrous, rough-surfaced, and composite materials, can severely disturb low-energy imaging. In order to image and quantify cell structures in optically challenging samples, we combined labeling, 3D X-ray imaging, and in silico processing into a methodological pipeline. Cell-structure images were acquired by a tube-source X-ray microtomography device and compared to optical references for assessing the visual and quantitative accuracy. The spatial coverage of the X-ray imaging was demonstrated by investigating stem-cell nuclei inside clinically relevant-sized tissue-engineering scaffolds (5x13 mm) that were difficult to examine with the optical methods. Our results highlight the potential of the readily available X-ray microtomography devices that can be used to thoroughly study relative large cell-cultured samples with microscopic 3D accuracy.
Kokoelmat
- TUNICRIS-julkaisut [16951]