Transcription closed and open complex formation coordinate expression of genes with a shared promoter region
Häkkinen, Antti; Oliveira, Samuel M.D.; Neeli-Venkata, Ramakanth; Ribeiro, Andre S. (2019-12-01)
Häkkinen, Antti
Oliveira, Samuel M.D.
Neeli-Venkata, Ramakanth
Ribeiro, Andre S.
01.12.2019
20190507
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:tuni-202012229103
https://urn.fi/URN:NBN:fi:tuni-202012229103
Kuvaus
Peer reviewed
Tiivistelmä
Many genes are spaced closely, allowing coordination without explicit control through shared regulatory elements and molecular interactions. We study the dynamics of a stochastic model of a gene-pair in a head-to-head configuration, sharing promoter elements, which accounts for the rate-limiting steps in transcription initiation. We find that only in specific regions of the parameter space of the rate-limiting steps is orderly coexpression exhibited, suggesting that successful cooperation between closely spaced genes requires the coevolution of compatible rate-limiting step configuration. The model predictions are validated using in vivo single-cell, single-RNA measurements of the dynamics of pairs of genes sharing promoter elements. Our results suggest that, in E. coli, the kinetics of the rate-limiting steps in active transcription can play a central role in shaping the dynamics of gene-pairs sharing promoter elements.
Kokoelmat
- TUNICRIS-julkaisut [19020]